Overproduction of E. coli xylose isomerase |
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Authors: | S. M. Lastick M. Y. Tucker V. Mackedonski K. Grohmann |
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Affiliation: | (1) Solar Energy Research Institute, 1617 Cole Blvd., 80401 Golden, CO |
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Abstract: | Summary A promoterles DNA fragment containing theE. coli xylose isomerase gene and its ribosome binding site was ligated into a plasmid downstream from the strong PL promoter. The plasmid was then used to transformE. coli strains containing a temperature-sensitive repressor (cI857). The transformants overproduced xylose isomerase when the repressor was thermally inactivated. |
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