Expression of cloned aromatic L-amino acid decarboxylase in Xenopus laevis oocytes. |
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Authors: | K P Gudehithlu A M Duchemin C P Silvia N H Neff M Hadjiconstantinou |
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Affiliation: | Department of Pharmacology, Ohio State University College of Medicine, Columbus 43210. |
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Abstract: | Sense mRNA coding for bovine adrenal medulla aromatic L-amino acid decarboxylase (AADC) was expressed following microinjection into Xenopus laevis oocytes. The expressed enzyme activity was stereoselective for L-5-hydroxytryptophan and L-DOPA and blocked by NSD-1015 an inhibitor of AADC. Heating the expressed enzyme at 55 degrees C resulted in a parallel loss of activity towards both substrates. Our findings are consistent with the prevailing notion that a single enzyme is able to decarboxylate both substrates in vivo. |
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