Characterization of arylsulfatase formed by derepressed synthesis in Citrobacter braakii |
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Authors: | Miura Toyokazu Okamoto Kenji Yanase Hideshi |
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Institution: | Department of Biotechnology, Faculty of Engineering, Tottori University. |
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Abstract: | Arylsulfatase activity was detected in a bacterial strain, Citrobacter braakii 69-b, isolated from soil by enrichment cultivation using porcine gastric mucin. The production of arylsulfatase was derepressed markedly in a synthetic medium by the addition of tyramine. The purified enzyme hydrolyzed 4-nitrophenyl sulfate, 4-nitrocatechol sulfate, and 3-indoxyl sulfate, and was classified as type I arylsulfatase. |
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