Crystallization and preliminary X-ray analysis of Sau3AI/E64A mutant protein |
| |
Authors: | Xu Chunyan Song Jiaping Ding Yu Yu Feng Sun Lihua Tang Lin Hu Xiaojian Zhang Zhihong He Jianhua |
| |
Institution: | Shanghai Institutes of Applied Physics, Chinese Academy of Sciences, 201800 Shanghai PR China. |
| |
Abstract: | Sau3AI is a type II restriction endonuclease that recognizes the palindromic sequence 5'-GATC-3' and cleaves 5' to G residue on each strand. The E64A mutant full length protein was cloned and expressed in Escherichia coli. The purified (His) (6)-tagged protein has monomer and dimer fraction and was crystallized by the hanging-drop vapor-diffusion technique. The dimer protein crystals can diffract to 3.0A. resolution and the monomer protein crystals can diffract to better than 2.8A. resolution. One completed dataset has been collected and it shows that the monomer orthorhombic Sau3AI/E64A crystal is in space group C2221 with unit cell parameters (69.44, 197.60, 191.46, 90, 90, 90) and contains two molecules in one asymmetric unit. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|