Cereulide-producing strains of <Emphasis Type="Italic">Bacillus cereus</Emphasis> show diversity |
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Authors: | Camelia?Apetroaie Maria?A?Andersson Cathrin?Spr?er Irina?Tsitko Ranad?Shaheen Elina?L?J??skel?inen Luc?M?Wijnands Ritva?Heikkil? Email author" target="_blank">Mirja?S?Salkinoja-SalonenEmail author |
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Institution: | (1) Department of Applied Chemistry and Microbiology, University of Helsinki, P.O. Box 56, Viikinkaari 9, 00014 Helsinki, Finland;(2) DSMZ Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, 38124 Braunschweig, Germany;(3) National Institute of Public Health and the Environment (RIVM), POB 1, 3720 B.A Bilthoven, The Netherlands;(4) Yhtyneet Laboratoriot Oy, PL 222, 00381 Helsinki, Finland |
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Abstract: | Producers of cereulide, the emetic toxin of Bacillus cereus, are known to constitute a specific subset within this species. We investigated physiological and genetic properties of 24
strains of B. cereus including two high cereulide producers (600–1,800 ng cereulide mg−1 wet weight biomass), seven average producers (180–600 ng cereulide mg−1 wet weight biomass), four low cereulide producers (20–160 ng cereulide mg−1 wet weight biomass) and 11 non-producers representing isolates from food, food poisoning, human gut and environment. The
13 cereulide producers possessed 16S rRNA gene sequences identical to each other and identical to that of B. anthracis strains Ames, Sterne from GenBank and strain NC 08234–02, but showed diversity in the adk gene (two sequence types), in ribopatterns obtained with EcoRI and PvuII (three types of patterns), in tyrosin decomposition, haemolysis and lecithin hydrolysis (two phenotypes). The cereulide-producing
isolates from the human gut represented two ribopatterns of which one was novel to cereulide-producing B. cereus and two phenotypes. We conclude that the cereulide-producing B. cereus are genetically and biochemically more diverse than hitherto thought. |
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Keywords: | Cereulide Bacillus cereus Ribopattern 16S rRNA gene Adk gene Lecithinase Haemolysis Tyrosine decomposition |
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