Overproduction of glutathione by l-cysteine addition and a temperature-shift strategy |
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Authors: | Gong-Yuan Wei Da-Hui Wang Jian Chen |
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Affiliation: | (1) School of Life Science, Soochow University, Suzhou, 215123, China;(2) Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214036, China |
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Abstract: | The present study investigated the effects of three constituent amino acids on glutathione production in flask culture of Candida utilis. Although l-glutamic acid and glycine had little impact on cell growth and glutathione biosynthesis, l-cysteine positively influenced glutathione production, despite inhibiting cell growth when it was added prior to stationary phase. Adding 8 mmol/L of l-cysteine to the culture broth at 16 h boosted glutathione production by 91%, increasing the intracellular glutathione content by 106% compared to untreated controls. A temperature-shift strategy, in which we shifted batch and fed-batch cultures of C. utilis from 30 to 26°C, also significantly enhanced glutathione production. Applying both strategies (i.e. adding 20 mmol/L l-cysteine and shifting the temperature from 30 to 26°C) at 33 h enhanced the glutathione concentration and the intracellular glutathione content to 1,312 mg/L and 3.75%, respectively, during fed-batch cultivation (glucose feeding at a constant rate of 18.3 g/h). The average specific glutathione production rate under this condition was 129% higher than that of the control without strategy. |
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Keywords: | Candida utilis glutathione font-variant:small-caps" >l-cysteine temperature-shift strategy |
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