Properties of [3H]taurine release from crude synaptosomal fractions of rat cerebral cortex |
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Authors: | P Placheta E Singer W Sieghart M Karobath |
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Institution: | (1) Institute of Pharmacology, University of Vienna, Austria;(2) Department of Biochemical Psychiatry, Psychiatrische Universitätsklinik, University of Vienna, Austria |
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Abstract: | The release of previously accumulated 3H]taurine and 14C]GABA from crude synaptosomal (P2) fractions isolated from rat cerebral cortex was studied using a superfusion system. The spontaneous efflux of 3H]taurine and 14C]GABA was stimulated by elevated concentrations of K+ (15–133 mM) in a concentration-dependent manner. This K+-stimulated release of 14C]GABA but not of 3H]taurine was enhanced in the presence of Ca2+. However, addition of 3 mM Ca2+ to the superfusion medium in the presence of the ionophore A 23187 resulted in a stimulation of the release of both 3H]taurine and 14C]GABA. These results are discussed in connection with the cellular localization of tourine in the central nervous system. |
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