The fatty acid profile of vegetative Azotobacter vinelandii ATCC 12837: growth phase-dependence |
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Authors: | A Larsen I Sliskovic D Juric C L Pinnock H Kullman E Segstro G Reinfelds M O Eze |
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Institution: | (1) Department of Chemistry, The University of Winnipeg, Winnipeg, Manitoba, R3B 2E9, Canada |
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Abstract: | Fatty acids of Azotobacter vinelandii ATCC 12837 were determined at various times during aerobic vegetative growth at 30°C to provide baseline data for studying
the effects of chemical agents on the organism’s survival and fatty acid biosynthesis. Palmitate (16:0) was the highest at
36.7±4.3 mol% (mean±SD) after the first 5 h in fresh culture, decreasing slightly to 33.4±2.6 mol% at 49 h. The other fatty
acids were therefore each normalized as a ratio of 16:0. At 5 h, as a ratio of 16:0, myristate (14:0) was 0.14±0.06, palmitoleate
(16:1cΔ9–10) 0.13±0.06, oleate (18:1cΔ9–10) 0.21±0.12, cis-vaccenate (18:1cΔ11–12) 0.30±0.17 and stearate (18:0) 0.68±0.02. As the growth phase advanced to 49 h, 14:0 and 16:1cΔ9–10 increased, 18:1cΔ9–10 decreased and cis-vaccenate reciprocally increased, whereas 18:0 decreased. These suggest that the saturated fatty acid biosynthesis pathway
yielded 16:0 and 18:0 in the 5-h lag period. By desaturation, 18:0 formed the unsaturated fatty acid (UFA) 18:1cΔ9–10. As the culture aged, the anaerobic UFA biosynthesis pathway formed 16:1cΔ9–10, which was elongated to 18:1cΔ11–12. These fatty acid alterations represent a homeoviscous adaptation, modulating the microbe’s membrane lipid viscosity for
optimal cellular function. |
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