(1) Laboratory of Biocatalysis, School of Biotechnology, Southern Yangtze University, 214036 Wuxi , P.R.China;(2) The Key Laboratory of Industrial Biotechnology, Ministry of Education, Wuxi, 214036, P.R.China
Abstract:
A d -lactonohydrolase gene of about 1.1 kb was cloned from Fusarium moniliforme. The ORF sequence predicted a protein of 382 amino acids with a molecular mass of about 40 kDa. An expression plasmid carrying the gene under the control of the triose phosphate isomerase gene promotor was introduced into Saccharomyces cerevisiae, and the d -lactonohydrolase gene was successfully expressed in the recombinant strains.Revisions requested 10 September 2004; Revisions received 15 October 2004The nucleotide sequence data reported in this paper has been assigned accession number AY728018 in the GeneBank database.
