Cloning and expression of <Emphasis Type="SmallCaps">d</Emphasis> -lactonohydrolase cDNA from <Emphasis Type="Italic">Fusarium moniliforme</Emphasis> in <Emphasis Type="Italic">Saccharomyces cerevisiae</Emphasis> |
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Authors: | Zhiqiang?Liu Email author" target="_blank">Zhihao?SunEmail author |
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Institution: | (1) Laboratory of Biocatalysis, School of Biotechnology, Southern Yangtze University, 214036 Wuxi , P.R.China;(2) The Key Laboratory of Industrial Biotechnology, Ministry of Education, Wuxi, 214036, P.R.China |
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Abstract: | A d -lactonohydrolase gene of about 1.1 kb was cloned from Fusarium moniliforme. The ORF sequence predicted a protein of 382 amino acids with a molecular mass of about 40 kDa. An expression plasmid carrying the gene under the control of the triose phosphate isomerase gene promotor was introduced into Saccharomyces cerevisiae, and the d -lactonohydrolase gene was successfully expressed in the recombinant strains.Revisions requested 10 September 2004; Revisions received 15 October 2004The nucleotide sequence data reported in this paper has been assigned accession number AY728018 in the GeneBank database. |
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Keywords: | cDNA cloning d-lactonohydrolase" target="_blank">d-lactonohydrolase eukaryotic expression Fusarium moniliforme |
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