Biochemical characteristics of the novel haloalkane dehalogenase DatA, isolated from the plant pathogen Agrobacterium tumefaciens C58 |
| |
| Authors: | Hasan Khomaini Fortova Andrea Koudelakova Tana Chaloupkova Radka Ishitsuka Mayuko Nagata Yuji Damborsky Jiri Prokop Zbynek |
| |
| Institution: | oschmidt Laboratories, Department of Experimental Biology and Research Centre for Toxic Compounds in the Environment, Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic. |
| |
| Abstract: | We report the biochemical characterization of a novel haloalkane dehalogenase, DatA, isolated from the plant pathogen Agrobacterium tumefaciens C58. DatA possesses a peculiar pair of halide-stabilizing residues, Asn-Tyr, which have not been reported to play this role in other known haloalkane dehalogenases. DatA has a number of other unique characteristics, including substrate-dependent and cooperative kinetics, a dimeric structure, and excellent enantioselectivity toward racemic mixtures of chiral brominated alkanes and esters. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
