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Luteinizing hormone response to different doses of synthetic gonadotropin-releasing hormone during early and late lactation in primiparous sows
Affiliation:1. DeSantis Distinguished Professor of Finance and Entrepreneurship, College of Business, Florida Atlantic University, 777 Glades Road, Boca Raton, Florida 33432 United States;2. Visiting Professor, Birmingham Business School, University of Birmingham, University House, 116 Edgebaston Park Rd., Birmingham, B15 2TY, United Kingdom;3. Capital Markets CRC Limited (CMCRC), 3/55 Harrington St, Sydney, NSW 2000, Australia;4. DeepR Analytics, Co-founder & CEO, 20 Bay St, Floor 11, Toronto, Ontario M3J 1P3, Canada;5. University of Exeter Business School, Streatham Court, Streatham Campus, Rennes Drive, Exeter EX4 4PU, United Kingdom;1. Laboratory of Medicinal and Environment Chemistry, University of Sfax, Higher Institute of Biotechnology, BP 261, 3000 Sfax, Tunisia;2. Laboratory of Molecular and Cellular Screening Processes, Centre of Biotechnology of Sfax, B.P. 1177, 3018 Sfax, Tunisia;3. Laboratory of Microorganisms and Biomolecules of the Center of Biotechnology of Sfax, Sfax, Tunisia;4. Laboratory of Biochemistry, CHU Habib Bourguiba, University of Sfax, Sfax, Tunisia;5. Regional Central of Blood Transfusion of Sfax, El-Ain Km 0.5, CP 3003 Sfax, Tunisia;6. Laboratory of Enzyme Engineering and Microbiology, National Engineering School of Sfax, University of Sfax, Tunisia;7. Laboratory of Anatomopathology, CHU Habib Bourguiba, University of Sfax, Sfax, Tunisia;8. Laboratory of Anatomy, Faculty of Medicine of Sfax and Department of Orthopedic and Traumatological Surgery, CHU Habib Bourguiba, Sfax, Tunisia;9. Laboratory of Materials Multifunctional and Applications (LaMMA, LR16ES18), Faculty of Sciences of Sfax, University of Sfax, BP 1171, 3000 Sfax, Tunisia
Abstract:The object of this investigation was to study the luteinizing hormone (LH) response to different doses of synthetic exogenous gonadotropin-releasing hormone (GnRH) during early (days 7–15) and late (days 20–28) lactation in primiparous sows. Five crossbred primiparous lactating sows were used in this study. The lactation period was 35 days. Five doses of GnRH, 0, 2.5, 5.0, 10.0 and 20.0μg were administered via a jugular vein catheter every second day in a latin square design during both the early and late lactation period. Daily (9.00 a.m.) blood samples were taken for oestradiol–17β and progesterone analysis. Frequent blood samples were taken before and after GnRH treatment for LH analysis. The total LH response was measured from post-treatment samples as the area under the curve above the base level, obtained from pre-treatment samples.No elevation of plasma oestradiol–17β or plasma progesterone occurred during lactation. The LH response increased with increasing doses of GnRH during early as well as late lactation. The total LH response during late lactation was a little greater than during early lactation, but the difference was not significant (P=0.055). There was no effect of the time sequence of treatment within phase of lactation.
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