Mechanism of adenosine production by xanthine-requiring mutants derived from a Bacillus strain |
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| Institution: | 1. Dermatology Research Centre, Institute of Inflammation and Repair, University of Manchester, Manchester, M13 9PT, UK;2. Department of Dermatology, University of Münster, D-48149 Münster, Germany;3. Department of Dermatology, University of Lübeck, D-23538 Lübeck, Germany;4. Department of Pediatrics, Paracelsus Medical University, A-5020 Salzburg, Austria;5. Department of Dermatology, Hadassah – Hebrew University Medical Center, Jerusalem, 9112001, Israel;6. Department of Biological Chemistry, University of California, Irvine, CA 92697, USA;7. Department of Medicine, Division of Endocrinology, University of California, Irvine, CA 92697, USA;8. Institute for Genomics and Bioinformatics, University of California, Irvine, CA 92697, USA;1. Institute of Clinical Medicine, University of Eastern Finland, P.O. Box 1627, 70211, Kuopio, Finland;2. Department of Psychiatry, Kuopio University Hospital, P.O. Box 100, 70029 KYS, Finland;3. Metabolomics Unit, Institute for Molecular Medicine, Finland;4. FIMM, P.O. Box 20, FI-00014, University of Helsinki, Finland;5. Primary Health Care Unit, University of Eastern Finland and Kuopio University Hospital, P.O. Box 1627, 70211, Kuopio, Finland;6. Department of Education and Psychology, University of Eastern Finland, P.O. Box 111, 80101 Joensuu, Finland |
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| Abstract: | The xanthine-requiring mutants defective in adenine deaminase (adenase) derived from a Bacillus strain accumulate much adenosine. The mechanism of adenosine production was investigated. Limitation of the guanine-related substances in the fermentation medium facilitated the adenosine accumulation, but the excess of those suppressed it.Metabolic regulation of the purine nucleotide biosynthesis was supposed to be released from both feedback inhibition and repression by limiting the concentration of guanine-related substances in the cells caused by xanthine-requirement. Deficiency in the deaminase activities of adenine, adenosine and AMP and the weak adenosine phosphorylase activity contributed to adenosine accumulation. No apparent changes were observed in the adenylosuccinate synthetase activity and the dephosphorylation activity of AMP compared with the wild strain. |
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