Effect of amino compounds on alkaline amylase production by alkalophilic Bacillus sp. |
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| Affiliation: | 1. Universidade de Passo Fundo (UPF) and Faculdade Meridional (IMED), Passo Fundo, Rio Grande do Sul (RS) and Chief of Allergy, Clinical Immunology, Department of Pediatrics Allergy and Immunology Pediatric at Universidade Federal da Fronteira Sul (UFFS), Brazil;2. Clinical Immunology and Rheumatology, Department of Pediatrics, Universidade Federal de São Paulo (UNIFESP), Escola Paulista de Medicina (EPM), São Paulo, SP, Brazil;3. Epidemiologist of the GPPG at the Hospital de Clínicas de Porto Alegre (HCPA), Porto Alegre, RS, Brazil;4. Universidade Federal da Fronteira Sul (UFFS), Passo Fundo, RS, Brazil;5. Faculdade Meridional (IMED), Passo Fundo, RS, Brazil;6. Pneumology Division at the HCPA – UFRGS, Porto Alegre, RS, Brazil;1. Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India;2. Malaria Research Group, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India;1. College of Food Science and Engineer of Jiangxi Agricultural University, Nanchang 330045, China;2. Key Lab for Agricultural Product Processing and Quality Control of Nanchang City, Nanchang 330045, China |
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| Abstract: | Of the amino compounds investigated, β-alanine, dl-norvaline and d-methionine were effective for the production of alkaline amylase by alkalophilic Bacillus no. A-40-2. The addition of 0.5% dl-norvaline and 0.5% d-methionine to the culture medium increased amylase production 1.7-fold, while they repressed growth slightly or strongly. There was no relation between amylase yield and the extracellular protein amount. Because alkaline protease activity was negligible in the culture fluid, these compounds might change the regulation of amylase synthesis and/or make amylase excretion easier. |
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