L-serine production by a methionine-auxotrophic mutant of methylotrophic Pseudomonas |
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Institution: | 1. a Universidad Autónoma de Yucatán. Campus de Ingenierías y Ciencias Exactas, Periférico Norte km 33.5, C.P. 97203 Mérida, Yucatán, Mexico;2. b Tecnológico Nacional de México/Instituto Tecnológico de Cancún, DEPI, Av. Kabah km 3, 77500 Cancún, Q. Roo, Mexico |
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Abstract: | A methionine-auxotropic mutant deficient in homocysteine transmethylation activity was induced from a methylotrophic L-serine-producing derivatives of Pseudomonas MS31. This mutant grown with limited L-methionine had more than 1.7-fold higher serine hydroxymethyltransferase (SHMT) activity than its parent strain. The elevated SHMT activity significantly contributed to the improvement of L-serine accumulation from glycine and methanol. Under the optimum conditions, this mutant accumulated up to 23.9 mg/ml of L-serine. The yield coefficient L-serine from consumed glycine was 89% (mol/mol). The maximum conversion rate of added glycine (19 mg/ml) to L-serine was 77% (mol/mol). |
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