Humic acid-vitamin agar,a new medium for the selective isolation of soil actinomycetes |
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| Institution: | 1. Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand;2. Interdisciplinary Graduate Program in Genetic Engineering, Graduate School, Kasetsart University, Bangkok 10900, Thailand;3. Center for Advanced Studies in Tropical Natural Resources, Kasetsart University, Bangkok 10900, Thailand;1. Mizoram University, Aizawl, Mizoram, India;2. Tallinn University of Technology, Tallinn, Estonia;1. Department of Microbiology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University, Lwowska 1, 87 100 Torun, Poland;2. School of Natural and Environmental Sciences, Ridley Building 2, Newcastle University, Newcastle upon Tyne NE1 7RU, United Kingdom;3. Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany;4. Nanobiotechnology Laboratory, Department of Biotechnology, SGB Amravati University, Amravati-444602, Maharashtra, India;5. Faculty of Health and Life Sciences, Northumbria University, Newcastle upon Tyne, United Kingdom;1. Medical Microbiology & Marine Pharmacology Unit, Department of Marine Science, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India;2. Laboratorio de Nanocelulosa y Biomateriales, Departamento de Ingeniería Química, Biotecnología y Materiales, Facultad de Ciencias Físicas y Matemáticas, Universidad de Chile, Avenida Beauchef 851, Santiago, Chile;1. Department of Biotechnology, Andhra University College of Pharmaceutical Sciences, Visakhapatnam 530013, Andhra Pradesh, India;2. Department of Pharmaceutical Analysis, Raghu College of Pharmacy, Dakamarri, Bheemunipatnam, Visakhapatnam 531162, Andhra Pradesh, India |
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| Abstract: | A new medium, designated HV agar, containing soil humic acid as the sole source of carbon and nitrogen was developed.The HV agar was superior to other currently used media, including colloidal chitin agar, glycerol-arginine agar and starch-casein-nitrate agar, for the isolation and enumeration of soil actinomycetes: It allowed the growth of the largest numbers of actinomycete colonies belonging to each genus of Streptomyces, Micromonospora, Microbispora, Streptosporangium, Nocardia, Dactylosporangium, Microtetraspora and Thermomonospora on the plate, while restricting the development of true bacteria. The HV agar supported adequate growth and good sporulation for these actinomycetes.Even when spore suspensions were used as the inoculum, the HV agar produced remarkably larger numbers of actinomycetes, especially strains of the genera Micromonospora, Microbispora, Streptosporangium, Dactylosporangium and Saccharomonospora, than did glycerol-arginine agar. It was found that the spores of these actinomycetes were activated upon germination by treatment at 20°C for 30 min with a O.2% solution of humic acid prior to incubation. |
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