HETP values of large-scale columns for liquid chromatography |
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| Affiliation: | 1. Department of Chemical and Process Engineering, Rzeszów University of Technology, Powstańców Warszawy Ave. 6, 35-959 Rzeszów, Poland;2. Austrian Centre of Industrial Biotechnology, Muthgasse 11, 1190, Vienna, Austria;3. Department of Biotechnology, University of Natural Resources and Life Sciences Vienna, Muthgasse 18, 1190, Vienna, Austria;1. Departamento de Física, Universidade Federal do Ceará, P.O. Box 6030, CEP 60455-900 Fortaleza, Ceará, Brazil;2. Faculdade de Física, Universidade Federal do Pará, Belém, Pará, Brazil;3. Departamento de Química, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil |
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| Abstract: | Two columns of 100 mm and 200 mm in diameter for bench-scale liquid chromatography were designed to obtain a small dead volume and an appropriately uniform distribution of liquid, and the values of the height equivalent to a theoretical plate were measured for three kinds of packing beads, Sepharose 4B, Cellulofine GC-700 m and Silica. A suitable pressure drop through a distribution plate and/or a packed bed helps to distribute liquid flow uniformly over the column diameter, and reasonable HETP values were obtained. |
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