Continuous blue pigment formation by gardenia fruit using immobilized growing cells |
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| Affiliation: | 1. Chongqing Key Lab of Natural Medicine Research, Chongqing Technology and Business University, Chongqing 400067, China;2. College of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing 400050, China;3. Faculty of Health Sciences, University of Macau, Taipa, Macau, China |
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| Abstract: | Bacillus subtilis no. 24 was used as a microorganism which hydrolyzes geniposide and forms a blue pigment. This microorganism possessed β-glucosidase activity during aerobic growth (log phase) and assimilated geniposide as a carbon source. The growth of this cell was depressed by genipin formed by the hydrolysis of geniposide. Blue pigment was formed continuously for 20 d in medium containing geniposide, yeast extract and Polypepton, using growing cells immobilized in calcium-alginate gel. |
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