Rheological behaviour of high density continuous cultures of Saccharomyces cerevisiae |
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| Affiliation: | 1. Rua São Francisco Xavier, Universidade do Estado do Rio de Janeiro (UERJ), 524, Maracanã, Rio de Janeiro, RJ CEP 20550-900, Brazil;2. Chemical Engineering Program, COPPE, Universidade Federal do Rio de Janeiro (UFRJ), CT, Bloco G, Ilha do Fundão, Rio de Janeiro, RJ CEP 21941-914, Brazil;3. School of Chemistry, Universidade Federal do Rio de Janeiro (UFRJ), CT, Bloco E, Ilha do Fundão, Rio de Janeiro, RJ CEP 21949-900, Brazil;4. School of Chemical, Biological and Materials Engineering, University of Oklahoma, Norman, OK 73019, USA |
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| Abstract: | By culture of Saccharomyces cerevisiae with cell recycle using tangential microfiltration, high cell concentrations are obtained (in the range of 0 to 345 gl−1 dry-weight). The rheological properties of the cell suspension during the cell growth were studied. Over a wide range of biomass concentration (X<275 gl−1D.W.) the power-law model was found adequate to describe the rheological behaviour of the broth. Pronounced non-Newtonian (pseudoplastic) behaviour occurred for X > 75 gl−1. Experimental correlations for apparent viscosity (na, mPa.s) and power-law index vs. biomass concentration (X, gl−1) were established: na = (1+0.012X)2 suitable over the whole range of concentration up to 275 gl−1 D.W. na = 1+0.04X in the low concentration range; X<100 gl−1D.W. Beyond the cell concentration of 275 gl−1 D.W. the viscosity increases suddenly. |
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