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Formaldehyde production with heat-treated cells of methanol yeast
Institution:1. Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University, Kyoto 606, Japan;1. Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University, Kyoto 606, Japan;1. The Ohio State University, Columbus, Ohio;2. Division of Endodontics, The Ohio State University, Columbus, Ohio;3. Division of Oral Biology, The Ohio State University, Columbus, Ohio;1. College of Food Science and Technology, Southwest Minzu University, Chengdu 610041, China;2. Department of Food Science and Engineering, College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, China;1. MARUM – Center for Marine Environmental Sciences, University of Bremen, Leobener Str. 8, 28359 Bremen, Germany;2. Division of Soil and Water Management, University of Leuven, Celestijnenlaan 200e, 3001 Leuven, Belgium;1. IES, Univ. Montpellier, CNRS, F-34000 Montpellier, France;2. CTM, Univ. Montpellier, F-34000 Montpellier, France;3. C2N, CNRS- Univ. Paris-Sud, Univ. Paris-Saclay, 10 Avenue Thomas Gobert, F-91120 Palaiseau, France
Abstract:Chemostat-grown cells of a methanol yeast, Candida boidini S2 AOU-1, were investigated for formaldehyde production. The productivity and catalytic stability were improved by preincubation of the cells at 37°C for 24 h in 0.1 M potassium phosphate buffer (pH 7.5) containing 50 mM NaN3. These cells produced 1150 mM formaldehyde after a 10-h reaction.
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