Microphotometric measurement of some enzymatic activities in rabbit spermatozoa during epididymal maturation |
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| Institution: | 1. Laboratório de Reprodução e Melhoramento Genético Animal, Centro de Ciências e Tecnologias Agropecuárias (CCTA), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, RJ, Brazil;2. Laboratório de Biotecnologia, Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, RJ, Brazil;3. Unidade de Biologia Integrativa, Setor de Genômica e Proteômica, Laboratório de Biotecnologia, Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, RJ, Brazil;1. Varisson Radiology Center, Yeşilbahçe Mah, Metin Kasapoğlu Cad Nuri Mancar Apt, D:No:42/A, 07160 Muratpaşa/Antalya, Turkey;2. Department of Nuclear Medicine, Memorial Bahcelievler Hospital, Istanbul, Turkey;3. Department of Endocrinology, Akdeniz University School of Medicine, Antalya, Turkey;1. Department of Poultry Production, Faculty of Agriculture, Assiut University, 71526 Assiut, Egypt;2. Departamento de Reproducción Animal, Instituto Nacional de Investigación y Tecnologia Agraria y Alimentaria (INIA), 28040 Madrid, Spain;1. The Ohio State University, College of Veterinary Medicine, Department of Veterinary Clinical Sciences, Columbus, OH, USA;2. The Ohio State University, College of Veterinary Medicine, Department of Veterinary Preventive Medicine, Columbus, OH, USA;3. The Ohio State University, College of Veterinary Medicine, Department of Veterinary Biosciences, Columbus, OH, USA;4. The Ohio State University, Department of Ecology, Evolution and Organismal Biology, Aquatic Ecology Lab, Columbus, OH, USA;5. The Ohio State University, College of Veterinary Medicine, Department of Veterinary Biosciences, Viruses and Emerging Pathogens Program, The Infectious Diseases Institute, Columbus, OH, USA;6. Colorado State University, College of Veterinary Medicine and Biomedical Sciences, Animal Reproduction and Biotechnology Laboratory, Fort Collins, CO, USA |
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| Abstract: | Cytochemical quantitative measurements of isocitrate dehydrogenase (ICDH), malate dehydrogenase (MDH), cytochrome oxidase, lactate dehydrogenase (LDH), glucose 6-phosphate dehydrogenase (G6PDH) and glutamate dehydrogenase (GLDH) activities were made on rabbit spermatozoa collected from the testis, the different epididymal sites and the ductus deferens. These measurements were made on individual spermatozoa (at least 100 spermatozoa for each site under consideration) using a Vickers M 85 scanning microdensitometer.The activity patterns of the enzymes involved in the tricarboxylic acid cycle (ICDH, MDH) and in the respiratory chain (cytochrome oxidase) both showed a progressive decrease in the intramitochondrial oxidative metabolism from the testis to the ductus deferens. This was in contrast to LDH activity which represents the anaerobic glycolysis pathway rather than the activity of intramitochondrial LDH. The G6PDH activity could be related to those membrane modifications which the male gamete undergoes during its epididymal maturation. Potential GLDH activity was relatively intense in the spermatozoa from the testis and from the initial and distal segments of the genital tract, suggesting an intramitochondrial synthesis of enzymes such as cytochrome oxidase or ATPase.The quantitative variations of the enzymatic activities occurring during the transit of spermatozoa along the male genital tract suggested the existence of different specific interactions between the spermatozoon and the epididymal microenvironment. |
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