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Expression in Escherichia coli: becoming faster and more complex

Authors:	Renaud Vincentelli Christophe Romier

Institution:	1. Architecture et Fonction des Macromolécules Biologiques (A.F.M.B), UMR7257 CNRS, Université Aix-Marseille, Case 932, 163 Avenue de Luminy, 13288 Marseille Cedex 9, France;2. Département de Biologie Structurale Intégrative, Institut de Génétique et Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UDS), CNRS, INSERM, 1 rue Laurent Fries, B.P. 10142, 67404 Illkirch Cedex, France

Abstract:	Download : Download high-res image (190KB) Download : Download full-size image Highlights? Escherichia coli: a highly versatile host to produce samples for structural biology. ? High-throughput technologies have increased single protein expression success rates. ? Co-expression eases production of complexes but still suffers from bottlenecks. ? Co-expression combined with high-throughput can tackle complex assemblies.

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