Cathepsin K knockout alleviates aging‐induced cardiac dysfunction |
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Authors: | Yinan Hua Timothy J. Robinson Yongtao Cao Guo‐Ping Shi Jun Ren Sreejayan Nair |
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Affiliation: | 1. Division of Pharmaceutical Sciences & Center for Cardiovascular Research and Alternative Medicine, School of Pharmacy, College of Health Sciences, Laramie, WY, USA;2. WWAMI Medical Education, College of Health Sciences, University of Wyoming, Laramie, WY, USA;3. Department of Mathematics, Indiana University of Pennsylvania, Indiana, PA, USA;4. Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA |
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Abstract: | Aging is a major risk factor for cardiovascular disease. It has previously been shown that protein levels of cathepsin K, a lysosomal cysteine protease, are elevated in the failing heart and that genetic ablation of cathepsin K protects against pressure overload‐induced cardiac hypertrophy and contractile dysfunction. Here we test the hypothesis that cathepsin K knockout alleviates age‐dependent decline in cardiac function. Cardiac geometry, contractile function, intracellular Ca2+ properties, and cardiomyocyte apoptosis were evaluated using echocardiography, fura‐2 technique, immunohistochemistry, Western blot and TUNEL staining, respectively. Aged (24‐month‐old) mice exhibited significant cardiac remodeling (enlarged chamber size, wall thickness, myocyte cross‐sectional area, and fibrosis), decreased cardiac contractility, prolonged relengthening along with compromised intracellular Ca2+ release compared to young (6‐month‐old) mice, which were attenuated in the cathepsin K knockout mice. Cellular markers of senescence, including cardiac lipofuscin, p21 and p16, were lower in the aged‐cathepsin K knockout mice compared to their wild‐type counterpart. Mechanistically, cathepsin K knockout mice attenuated an age‐induced increase in cardiomyocyte apoptosis and nuclear translocation of mitochondrial apoptosis‐inducing factor (AIF). In cultured H9c2 cells, doxorubicin stimulated premature senescence and apoptosis. Silencing of cathepsin K blocked the doxorubicin‐induced translocation of AIF from the mitochondria to the nuclei. Collectively, these results suggest that cathepsin K knockout attenuates age‐related decline in cardiac function via suppressing caspase‐dependent and caspase‐independent apoptosis. |
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Keywords: | aging apoptosis cardiac remodeling cathepsin K cardiac function cardiac hypertrophy |
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