| 饲喂肉杆菌Hg4-03对贡嘎蝠蛾幼虫肠菌生物多样性的影响 |
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| 引用本文： | 穆冬冬,王中康,殷幼平.饲喂肉杆菌Hg4-03对贡嘎蝠蛾幼虫肠菌生物多样性的影响[J].微生物学报,2010,50(2):251-255. |
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| 作者姓名： | 穆冬冬 王中康 殷幼平 |
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| 作者单位： | 重庆大学生物工程学院基因工程研究中心,重庆市功能基因及调控技术重点实验室,重庆,400030 |
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| 基金项目： | 国家自然科学基金（30572325） |
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| 摘 要： | 【目的】研究贡嘎蝠蛾幼虫肠道优势菌肉杆菌(Carnobacterium sp.)Hg4-03作为食物添加剂对实验室饲养的4龄贡嘎蝠蛾幼虫肠道菌群的影响。【方法】采用16S rDNA序列与PCR/DGGE(Denaturing gradient gel electrophoresis)分析技术相结合的方法,健康幼虫被随机分为处理组1、处理组2和对照组,两组处理组分别饲喂添加不同浓度肉杆菌Hg4-03的天然饲料,对照组只饲喂天然饲料。14 d和28 d后每组随机解剖6条幼虫,收集肠道样品,经细菌通用引物扩增细菌16S rDNA,DGGE分离并进行细菌多样性图谱分析。【结果】饲喂肉杆菌Hg4-03后幼虫肠道菌群的多样性指数呈上升趋势;处理组幼虫肠道中肉杆菌Hg4-03含量增加,且处理组中枯草芽孢杆菌(Bacillus subtilis)的量也明显增加。【结论】将肉杆菌Hg4-03作为益生菌饲喂贡嘎蝠蛾幼虫有助于维持幼虫肠道菌群多样性平衡,这为贡嘎蝠蛾人工或半人工养殖提供了一定的参考价值。
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| 关 键 词： | 关键词：PCR/DGGE 贡嘎蝠蛾幼虫 肉杆菌 Hg4-03 肠道菌群 益生菌添加 |
| 收稿时间： | 2009/8/20 0:00:00 |
| 修稿时间： | 2009/9/25 0:00:00 |
Changes of intestinal microflora in Hepialus gonggaensis larvae after feeding with Carnobacterium sp.Hg4-03 as a probiotic strain |
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| Dongdong Mu,Zhongkang Wang and Youping Yin.Changes of intestinal microflora in Hepialus gonggaensis larvae after feeding with Carnobacterium sp.Hg4-03 as a probiotic strain[J].Acta Microbiologica Sinica,2010,50(2):251-255. |
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| Authors: | Dongdong Mu Zhongkang Wang and Youping Yin |
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| Abstract: | Objective] We investigated the influence of Carnobacterium sp.Hg4-03 on intestinal microflora of the 4th instar Hepialus gonggaensis larvae,an economically important insect.Methods] The effect was observed by molecular techniques based on the sequence of 16S rDNA V3 region gene and PCR/DGGE(Denaturing gradient gel electrophoresis).The health larvae were divided randomly into treatment group1,treatment group2 and control group,and fed on their natural food added with different amount of Carnobacterium sp.H... |
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| Keywords: | Keywords: PCR/DGGE Hepialus gonggaensis larvae Carnobacterium sp Hg4-03 intestinal bacteria feeding probiotic |
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