应用SRAP标记绘制88份南瓜属种质资源DNA指纹图谱

为了给南瓜属种质资源鉴定和分类提供分子生物学依据,本研究采用SRAP分子标记技术与DNAMAN指纹图谱绘制软件对88份南瓜属种质资源(包含美洲南瓜、中国南瓜、印度南瓜)进行分子指纹图谱绘制。结果表明:35对SRAP多态性引物共扩增出499条清晰条带,其中多态性条带438条,多态性条带比率高达87.8%。根据扩增出的条带成功绘制出88份南瓜属种质资源的DNA指纹图谱,每一份种质都具有其独特的分子身份证,使得每份种质均可被区别开来。其中,多态性最好的引物是E5EM8,可以同时绘制72份南瓜属种质资源的指纹图谱。所有供试材料用5对多态性SRAP引物即可全部区别开来。研究表明,SRAP分子标记技术可成功地绘制南瓜属种质资源DNA指纹图谱。本研究对南瓜属种质资源鉴别、分子数据库构建及品种权保护具有较重要的意义。

Construction of Molecular Fingerprinting Map in Cucurbita genus including Spaghetti Squash by SRAP Markers

In order to provide molecular foundation for identification of Cucurbita genus germplasm, in present study, the molecular fingerprints of88 accessions of Cucurbita genus includingC. pepo. L, C. moschata. L and C. maxima.L were mapped using SRAP (Sequence Related Amplified Polymorphism) molecular markers technology and DNAman software. The results are as follow: the genome DNA of 88 Cucurbita genus germplasm were amplified by 35 pairs of SRAP primers selected from 200 pairs of primers. 499 bands were got totally, of which 438 were polymorphic bands, and the rate of polymorphic bands reached as high as 87.8%. DNA fingerprints of all these 88 Cucurbita genus germplasm were well constructed according to the amplified bands, and a unique molecular ID card was mapped for each species so that it can be identified successfully. Furthermore, the primer E5EM8, which has the best polymorphism, can distinguish 72 Cucurbita genus accession thoroughly. All the 88 Cucurbita genus germplasm could be identified using 5 pairs of SRAP primers. The results also showed DNA fingerprints of Cucurbita genus germplasm can be constructed successfully by SRAP molecular markers technique. This study has significance on identifying the accessions, constructing molecular database and protecting cultivar patent of Cucurbita genus.