An embryogenic cell line of maize from A188 (Minnesota) contains Mu1-like elements |
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| Authors: | Robert V. Masterson K. Biagi J. G. Wheeler J. Stadler D. W. Morris |
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| Affiliation: | (1) Department of Genetics, Iowa State University, 50011 Ames, IA, USA;(2) Present address: Max Planck Institute, D-5000 Cologne 30, FRG;(3) Present address: Department of Biological Sciences, George Washington University, 20052, Washington, DC, USA |
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| Abstract: | The maize inbred line A188 is popularly used for the production of embryogenic cell lines. A188, maintained at the University of Minnesota, was found upon molecular analysis to contain 2 to 4 copies of a DNA sequence very similar in structure to transposable Mu1 elements, which have been implicated in Robertson's Mutator system. These Mu1-like elements are in the same chromosomal locations in sibling plants and in A188 cell cultures derived from them. This suggests that the elements are in an inactive state and do not undergo transposition. However, we have observed that they are not modified at the target sites for certain restriction endonucleases. Possible causes for the apparent lack of transposition of these Mu1-like elements in these A188 lines are discussed. Inasmuch as the elements do not transpose, they must be maintained in this line as homozygous Mendelian elements by self-pollination.Journal paper no. J-12269 of the Iowa Agriculture and Home Economics Experiment Station, Ames, Iowa 50011. Project 2707. |
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| Keywords: | embryogenic cell culture maize Mutator transposable elements |
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