Expression, purification, characterization and crystallization of flap endonuclease-1 from Methanococcus jannaschii |
| |
Authors: | Bae K W Baek K W Cho C S Hwang K Y Kim H R Sung H C Cho Y |
| |
Institution: | Structural Biology Center, Korea Institute of Science & Technology, Seoul. |
| |
Abstract: | A gene coding for a protein homologous to the flap endonuclease-1 (FEN-1) was cloned from Methanococcus jannaschii, overexpressed, purified and characterized. The gene product from M. jannaschii shows 5' endo-/exonuclease and 5' pseudo-Y-endonuclease activities as observed in the FEN-1 in eukaryotes. In addition, Methanococcus jannaschii FEN-1 functions effectively at high concentrations of salt, unlike eukaryotic FEN-1. We have crystallized Methanococcus jannaschii FEN-1 and analyzed its preliminary character. The crystal belongs to the space group of P2(1) with unit cell dimensions of a = 58.93 A, b = 42.53 A, c = 62.62 A and beta = 92.250. A complete data set has been collected at 2.0 A resolution using a frozen crystal. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|