Flagellum-mediated motility in Pelotomaculum thermopropionicum SI |
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Authors: | Tomoyuki Kosaka Mutsumi Goda Manami Inoue Toshiharu Yakushi Mamoru Yamada |
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Affiliation: | 1. Department of Biological Chemistry, College of Agriculture, Graduate school of Science and Technology for Innovation, Yamaguchi University, Yamaguchi, Japan;2. Research Center for Thermotolerant Microbial Resources, Yamaguchi University, Yamaguchi, Japan |
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Abstract: | The basic functions of a propionate-oxidizing bacterium Pelotomaculum thermopropionicum flagellum, such as motility and chemotaxis, have not been studied. To investigate its motility, we compared with that of Syntrophobacter fumaroxidans, an aflagellar propionate-oxidizing bacterium, in soft agar medium. P. thermopropionicum cells spread, while S. fumaroxidans cells moved downward slightly, indicating flagellum-dependent motility in P. thermopropionicum SI. The motility of P. thermopropionicum was inhibited by the addition of carbonyl cyanide m-chlorophenyl hydrazone, a proton uncoupler, which is consistent with the fact that stator protein, MotB of P. thermopropionicum, shared sequence homology with proton-type stators. In addition, 5-N-ethyl-N-isopropyl amiloride, an Na+ channel blocker, showed no inhibitory effect on the motility. Furthermore, motAB of P. thermopropionicum complemented the defective swimming ability of Escherichia coli ?motAB. These results suggest that the motility of P. thermopropionicum SI depends on the proton-type flagellar motor. |
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Keywords: | Motility flagellum propionate-oxidizing bacteria MotB-like protein proton-driven type |
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