Effects of dietary protein contents and habitual endurance exercise on supplemental leucine oxidation in mice |
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| Authors: | Hirokazu Taniguchi Nao Akiyama |
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| Affiliation: | 1. Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, Japan;2. Faculty of Agriculture, Ryukoku University, Shiga, Japan;3. Faculty of Agriculture, Ryukoku University, Shiga, Japan |
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| Abstract: | The effects of dietary protein contents and regular exercise on the oxidation of supplemented leucine were examined. In the short-term study, male BALB/cCrSlc mice were fed diets containing 0, 10, 20, 35, and 60% protein: energy ratios for 1 week. In the long-term study, exercised and sedentary mice were fed diets containing 20, 35, and 60% protein ratios for 9 weeks. After the feeding periods, the mice were a bolus administered oral supplements of l-[1-13C] leucine. Expired gas was analyzed, and oxidized leucine was expressed as a relative 13CO2/12CO2 ratio. In the short-term study, the peak 13CO2/12CO2 ratio significantly increased with diet protein concentrations. Moreover, the long-term study also showed that the peak 13CO2/12CO2 ratio was significantly increased by high protein diets in both exercised and sedentary mice. Our results indicate that supplemental leucine oxidation is associated with consumption of a high-protein diet, irrespective of exercise status. Abbreviations: AUC: area under the curve; EX: exercise; RQ: respiratory quotient; SED: sedentary; VO2/W: oxygen uptake per body weight |
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| Keywords: | Leucine supplementation leucine oxidation high-protein diet endurance exercise mass spectrometric analysis |
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