Gene cloning of the pink-colored protein from Pleurotus salmoneostramineus (PsPCP) and its species-specific chromoprotein are effective for colorization of the fruit body |
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Authors: | Yasuhisa Fukuta Kengo Kamei Aoi Matsui Yosuke Fuji Hiroki Onuma Norifumi Shirasaka |
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Affiliation: | Department of Applied Biological Chemistry, Faculty of Agriculture, Kindai University, Nara, Japan |
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Abstract: | Pleurotus salmoneostramineus is a pink mushroom. This pink color is a protein and forms a complex with 3H-indol-3-one. The gene encoding the pink-colored protein from P. salmoneostramineus (PsPCP) was cloned, and its sequence was elucidated as a 681-bp. The ORF encodes 226 amino acid residues. The amino acid sequence of the protein did not show any significant homology in the DDBJ/EMBL/GenBank databases. Recombinant PsPCP was expressed as the soluble form in E. coli. The reaction mixture of purified recombinant PsPCP and 3H-indol-3-one showed a pink color as the native pigment. A real-time PCR analysis revealed the strong expression of PsPCP in the primordium formation stage of the life cycle of the fungus; however, its expression decreased with the maturation of the fruit body. A comparison of PsPCP gene expression profiles between two strains revealed high levels in the dark-colored strain. |
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Keywords: | Pleurotus salmoneostramineus chromoprotein pink-colored protein real-time PCR |
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