1H Quantitative NMR analyses of β-asarone and related compounds for quality control of Acorus rhizome herbal drugs in terms of the effects of their constituents on in vitro acetylcholine esterase activity |
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Authors: | Nwe Haymar Lynn Thein Zaw Linn Cui Yanmei Yuuki Shimozu Shoko Taniguchi |
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Institution: | 1. Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan;2. Department of Food and Drug Administration, Ministry of Health and Sports, Nay Pyi Taw, Myanmar;3. Department of Traditional Medicine, Ministry of Health and Sports, Nay Pyi Taw, Myanmar |
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Abstract: | We used quantitative nuclear magnetic resonance analyses to measure the contents of major constituents of Acorus rhizome materials used as herbal drugs. The inhibitory effects of crude n-hexane extracts and their individual constituents on in vitro acetylcholine esterase activity were evaluated. The crude extracts had unexpectedly weak inhibitory effects (46–64% inhibition at 1.0 mg/mL), despite the high content (46–64%) of β-asarone, which independently had a potent effect (IC50 2.9 µM 0.61 µg/mL]). Further investigation revealed participation of eudesmin A, a lignan constituent, in the suppression of the inhibitory effect of β-asarone. |
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Keywords: | Acorus β-asarone eudesmin A acetylcholine esterase inhibitory activity qNMR |
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