Genomic map of Clostridium perfringens strain 13 |
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| Authors: | Shimizu T Ohshima S Ohtani K Shimizu T Hayashi H |
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| Institution: | Department of Microbiology, Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, Japan. tshimizu@md.tsukuba.ac.jp |
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| Abstract: | A physical and genetic map of Clostridium perfringens strain 13 was constructed. C. perfringens strain 13 was found to have a 3.1-Mb chromosome and a large 50-kb plasmid, indicating that strain 13 has a relatively small genome among C. perfringens strains. A total of 313 genetic markers were mapped on the chromosome of strain 13. Compared with the physical and genetic map of C. perfringens CPN50, strain 13 had a quite similar genome organization, but with a large deletion (approximately 400 kb) in a particular segment of the chromosome. Among several toxin genes, a beta2 toxin gene that is a novel virulence gene in C. perfringens was found to be located on the 50-kb plasmid. |
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| Keywords: | clostridium perfringens genome map genome organization pulsed field gel electrophoresis virulence plasmid |
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