Metabolism of 2,5-dichlorobenzoic acid inPseudomonas stutzeri |
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| Authors: | S A Kozlovsky G M Zaitsev F Kunc |
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| Institution: | (1) UNESCO-ROSTE Course, Institute of Microbiology, Academy of Sciences of the Czech Republic, 142 20 Prague 4, Czech Republic;(2) Institute of Microbiology, Academy of Sciences of the Belorussia, 220 067 Minsk, Belorussia;(3) Institute of Microbiology, Academy of Sciences of the Czech Republic, 142 20 Prague 4, Czech Republic |
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| Abstract: | 4-Chroropyrocatechol is formed as a results of the oxidation of 2,5-dichlorobenzoate byPseudomonas stutzeri. 3-Chloro-cis,cis-muconic acid is the product of the oxidation of 4-chloropyrocatechol. Pyrocatechol 1,2-dioxygenase, gentisate 1,2-dioxygenase,
but not pyrocatechol 2,3-dioxygenase or protocatechuate 3,4-dioxygenase activities were found in cell-free extracts. Theortho cleavage activity for catechols appeared to involve induction of isoenzymes with different stereospecificity towards chlorocatechols.
A catablic pathway for the degradation of 2,5-dichlorobenzoate by a newly isolated strain ofP. stutzeri was proposed. |
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