香附子块茎试管苗繁殖

以香附子块茎为材料，用组织培养的方法，进行试管苗繁殖研究。结果表明，1/3MS+炉灰渣培养基是块茎生长芽培养的适宜培养基；1/2MS+ZT 0.6 mg/L+NAA 0.1 mg/L是生长芽分化和继代分化繁殖培养的适宜培养基；1/2MS+ABT 2号6 mg/L+IAA 0.4 mg/L是生根和试管苗微型块茎培养的适宜培养基。培养30 d试管苗移栽成活率96%；培养60 d试管苗移栽成活率89.7%；种植的块茎发芽率99.8%；试管苗和微型块茎定植的成活率在99%以上；定植试管苗保持了香附子的植物学特征。

The Study on Tuber and Tube Seedlings Breeding of Cyperus rotundus

With the method of tissue culture, the tubers of Cyperus rotundus were used as materials to do the research on tube seedlings’ propagation. The results demonstrated that 1/3MS with the matrix of cinder was the ideal medium for adventitious buds growth, and 1/2MS+ZT 0.6 mg/L+NAA 0.1 mg/L was the optimum medium for differentiation cultivation and subculture of growth buds. The ideal medium for rooting and miniature tuber cultivation was 1/2MS+ABT2 6.0 mg/L+ IAA 0.4 mg/L. The transplanting survival rate of tube seedlings after 30 days’ training and 60 days’ training were 96% and 89.7%. The sprout growth rate of tubers was 99.8%. The planting survival rate of tube seedlings and miniature tubers were above 99%. The tube seedlings stable planted maintained all botany characteristics of the wild Cyperus rotundus.