GENOTRACE: cDNA-based local GENOme assembly from TRACE archives |
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Authors: | Berezikov Eugene Plasterk Ronald H A Cuppen Edwin |
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Affiliation: | Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands. |
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Abstract: | GENOTRACE identifies the genomic organization for a cDNA using raw data from genome sequencing projects in progress (trace archives). Local genomic contigs are generated, allowing for example the design of PCR primers in intronic sequences to amplify coding regions of a gene, needed for example for mutation or SNP detection. AVAILABILITY: The package and examples of output files can be downloaded from http://rat.niob.knaw.nl/GENOTRACE |
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