| 增加复制起始区对基因表达的影响 |
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| 引用本文： | 王二力,姚国伟.增加复制起始区对基因表达的影响[J].工业微生物,1995,25(2):1-4,32. |
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| 作者姓名： | 王二力 姚国伟 |
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| 作者单位： | 华东理工大学基因工程研究室 |
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| 摘 要： | 我们在质粒ｐｕＢ１１０的基础上组建了ｐＤＲ质粒，它们具有双复制起始区而只有一个抗卡那霉素基因。携带了这些质粒的宿主细胞对卡那霉素的抗性明显高于亲本株（Ｂ，ｓｕｂｔｉｌｉｓ １５０（ｐｕＢ１１０））。经限制性酶切图谱分析新获得的转化株具有二个复制起始区及一个 Ｋｍ＆４基因。说明增加复制起始区是提高重组子表达能力的途径之一。
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| 关 键 词： | 复制起始区 基因表达 |
Improvement of Expression Level by Increasing Number of Replication Origins in Piasmid |
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| Wang Erli,Yao Guowei,Zhang Xiaohui,Cao Xueying.Improvement of Expression Level by Increasing Number of Replication Origins in Piasmid[J].Industrial Microbiology,1995,25(2):1-4,32. |
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| Authors: | Wang Erli Yao Guowei Zhang Xiaohui Cao Xueying |
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| Abstract: | A plasmid pDR carrying two replication regions and only one resistant to kanamycin gene from the plasmid pUB 110 was constructed. The host presented a higher level of kanamycin gene expression than that of the original strain B. subtilis 150(pUB110). The restrictive map of plasmid pDR was determined and its characterization of resistance to Km showed that the improvement of the expression level could also be achieved by increasing the number of replication origins. |
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| Keywords: | Replication origin Gene expression |
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