Direct evidence for de novo synthesis of rat liver phenylalanine: pyruvate transaminase after glucagon treatment. |
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Authors: | J C Shih Y L Chan |
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Institution: | School of Pharmacy, University of Southern California, Los Angeles, California 90033 U.S.A. |
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Abstract: | A specific antibody to phenylalanine:pyruvate transaminase has been used to show that the number of enzyme molecules and the rate of enzyme synthesis are increased by glucagon and N6,O2′-dibutyryl cyclic AMP. Cycloheximide given simultaneously with glucagon or dibutyryl cyclic AMP blocked the increase in 3H]leucine incorporation when it was injected along with glucagon, but had no effect when given 4 h after the glucagon. This finding suggests that the mRNA synthesis for phenylalanine:pyruvate transaminase may be completed in 4 h. |
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Keywords: | To whom correspondence should be addressed |
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