A peptide antibody for rapid screening of Streptomyces species producing phospholipase D |
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Authors: | Uhm Tai-Boong Lee Sun Hee |
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Affiliation: | (1) Faculty of Biological Sciences and Institute for Molecular Biology and Genetics, Chonbuk National University, Jeonju, 561-756, Republic of Korea |
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Abstract: | By examining the conserved regions in the protein sequences of eight different Streptomyces phospholipase Ds (PLD) reported so far and the X-ray crystallographic structure of a Streptomyces PLD, we designed a peptide sequence, DPANRGAVGSGGYSQIKSL, for the screening of microorganisms producing PLD. In the enzyme-linked immunosorbent assay using a mouse antibody raised against the designed peptide, we recovered seven producing strains out of 128 soil isolates. |
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Keywords: | antibody phospholipase D screening Streptomyces |
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