Functional identification of cytokinesis-related genes from tobacco BY-2 cells |
| |
Authors: | Yi Yu Hai-Yun Wang Li-Na Liu Zhi-Ling Chen Gui-Xian Xia |
| |
Institution: | (1) National Center for Plant Gene Research, National Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100080, China;(2) Graduate School of Chinese Academy of Sciences, Beijing, 100049, China;(3) College of Life Science, Capital Normal University, Beijing, 100037, China |
| |
Abstract: | The molecular mechanisms controlling cytokinesis in plant cell division cycle remains largely unknown. In this study, a functional
approach was taken to identify genes that may play roles in cytokinesis in tobacco BY-2 cells, using fission yeast as the
host organism. A total of 22 BY-2 genes that perturbed the terminal stage of cell division when ectopically expressed in yeast
cells were isolated, among which, several encode for uncharacterized genes. Additionally, RT-PCR analysis indicated that four
of the isolated genes were expressed in a cell cycle-dependent manner. Our results demonstrate that fission yeast system can
be efficiently used to identify the genes that may function, either positively or negatively, in the regulation of cytokinesis.
More importantly, the candidate genes we have isolated in this work can provide useful information for unraveling the regulators
controlling cell separation at the late stage of BY-2 cell division.
Yi Yu and Hai-Yun Wang contributed equally to this work. |
| |
Keywords: | Tobacco BY-2 cells Cell cycle Cytokinesis Schizosaccharomyces pombe |
本文献已被 PubMed SpringerLink 等数据库收录! |
|