Optimization and elucidation of interactions between ammonium, nitrate and phosphate inCentella asiatica cell culture using response surface methodology |
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Authors: | Rozita Omar M A Abdullah M A Hasan M Marziah M K Siti Mazlina |
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Institution: | (1) Department of Chemical and Environmental Engineering, Faculty of Engineering, Universiti Putra Malaysia, Perak, Malaysia;(2) Department of Bioprocess Technology, Faculty of Biotechnology and Molecular Science, Universiti Putra Malaysia, Perak, Malaysia;(3) Department of Chemical Engineering, Universiti Teknologi Petronas, Perak, Malaysia;(4) Department of Biochemistry, Faculty of Biotechnology and Molecular Science, Universiti Putra Malaysia, Selangor, Malaysia |
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Abstract: | The effects of macronutrients (NO3
−, NH4
+ and PO4
3−) on cell growth and triterpenoids production inCentella asiatica cell suspension cultures were analyzed using the Box-Behnken response surface model experimental design. In screening and
optimization experiments, PO4
3− as a single factor significantly influenced cell growth where increasing the phosphate level from 0.1 to 2.4 or 2.6 mM, elevated
cell growth from 3.9 to 14–16 g/L. The optimum values predicted from the response surface model are 5.05 mM NH4
+, 15.0 mM NO3
− and 2.6 mM PO4
3−, yielding 16.0 g/L cell dry weight with 99% fitness to the experimental data. While the NH4
+-NO3
− interaction influenced cell growth positively in the optimization experiment, NH4
+ and NO3
− as single factors; and interactions of NO3
−-PO4
3−, NH4
+-PO4
3− and NH4
+-NO3
− were all negative in the screening experiment. Cell growth and the final pH level were positively affected by PO4
3−, but negatively affected by NH4
+ and NH4
+-PO4
3− interactions. The different effects of factors and their interactions on cell growth and final pH are influenced by a broad
or narrow range of macronutrient concentrations. The productions of triterpenoids however were lower than 4 mg/g cell dry
weight. |
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Keywords: | response surface methodology Centella asiatica optimization plant cell culture modelling fermentation |
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