| 脂肽的分离纯化与结构研究 |
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| 引用本文： | 吕应年,杨世忠,牟伯中. 脂肽的分离纯化与结构研究[J]. 微生物学通报, 2005, 32(1): 67-73 |
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| 作者姓名： | 吕应年 杨世忠 牟伯中 |
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| 作者单位： | 华东理工大学化学系,上海,200237 |
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| 基金项目： | 国家自然科学基金资助项目(No.59974014)，高等学校博士学科点专项科研基金资助课题(No.20030251002)，上海市教委资助项目~~ |
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| 摘 要： | 采用酸沉淀分离、有机溶剂提取、分级沉淀、脱色吸附及制备薄板的方法，从枯草芽孢杆菌HSO121的培养液中分离得到一种脂肽类化合物。茚三酮试验、红外光谱分析表明该脂肽具有环状结构，电喷雾离子化质谱结果显示，该脂肽是分子量为1，022D和1，036D的两个同系物，氨基酸分析及串联质谱分析表明，脂肽的肽链结构为Leu-Leu-Asp-Val-Leu-Leu-Glu，说明它是Surfactin的结构类似物。
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| 关 键 词： | 枯草芽孢杆菌 脂肽 分离纯化 环状结构 |
| 文章编号： | 0253-2654(2005)01-0067-07 |
| 修稿时间： | 2004-04-23 |
Isolation and Identification of a Lipopeptide |
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| LV Ying-Nian,YANG Shi-zhong,MU Bo-Zhong. Isolation and Identification of a Lipopeptide[J]. Microbiology China, 2005, 32(1): 67-73 |
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| Authors: | LV Ying-Nian YANG Shi-zhong MU Bo-Zhong |
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| Affiliation: | LV Ying-Nian YANG Shi-Zhong MU Bo-Zhong~ |
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| Abstract: | A lipopeptide compound was isolated from the culture of Bacillus subtilis HSO121 by methods of acidic precipitation, solvent extract, fractional precipitation, adsorption and prepared thin-layer chromatography; and its molecular structure was determined by by ninhydrin assay and IR methods following the Amino analysis, MS-MS and ESI-MS. It shows that the isolated lipopeptide consists of two homologues with molecular mass 1,022D and 1,036D and bearing a cyclic structure with the amino acid sequence Leu-Leu-Asp-Val-Leu-Leu-Glu in the peptide chain, which indicates that the isolated lipopeptide falls into the analogs of surfactin. |
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| Keywords: | Bacillus subtilis Lipopeptide Isolation Cyclic structure |
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