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Chloroplast tRNAAsp: nucleotide sequence and variation of in vivo levels during plastid maturation |
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| Authors: | Astrid Schön Simon Gough Dieter Söll |
| Institution: | (1) Department of Molecular Biophysics and Biochemistry, Yale University, P.O. Box 6666, 06511 New Haven, CT, USA;(2) Department of Physiology, Carlsberg Laboratories, Gamle Carlsberg Vej, Copenhagen, Denmark;(3) Present address: Laboratorium für Biochemie, Universität Bayreuth, Postfach 101251, 8580 Bayreuth, Germany |
| Abstract: | Two chloroplast tRNAAsp species from barley were purified by chromatography on benzoylated DEAE-cellulose and sequenced. They differ in the modification at position 34, where queuosine (Q) is present in one of the species. The same chromatographic procedure yielded only one tRNAGlu species, corroborating the assumption that the same tRNAGlu species participates in both protein and chlorophyll biosynthesis. The level of tRNAGlu remains unchanged after light treatment of etiolated seedlings, whereas the amount of tRNAAsp decreases to about 50% relative to the level of dark-grown plants. |
| Keywords: | chloroplast |
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