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Simultaneous monitoring of independent gene expression patterns in two types of cocultured fibroblasts with different color-emitting luciferases |
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| Authors: | Takako Noguchi Masaaki Ikeda Yoshihiro Ohmiya Yoshihiro Nakajima |
| Institution: | (1) Cell Dynamics Research Group, Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan;(2) Molecular Clock Project, Research Center for Genomic Medicine, Saitama Medical School, Yamane, Hidaka Saitama, 350-1241, Japan;(3) Department of Physiology, Saitama Medical School, Morohongo, Moroyama Saitama, 350-0495, Japan;(4) Division of Molecular/Cell Imaging Department of Photobiology, Hokkaido University Graduate School of Medicine, West 7, North 15, Kita-ku, Sapporo 060-8638, Japan |
| Abstract: | BackgroundLuciferase assay systems enable the real-time monitoring of gene expression in living cells. We have developed a dual-color luciferase assay system in which the expression of multiple genes can be tracked simultaneously using green- and red-emitting beetle luciferases. We have applied the system to monitoring independent gene expressions in two types of cocultured fibroblasts in real time. |
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