Purification of indole-3-acetic acid in plant extracts by immunoaffinity chromatography |
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| Affiliation: | 1. Innovation Center of Pesticide Research, Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, China;2. State Key Laboratory of the Discovery and Development of Novel Pesticide, Shenyang Sinochem Agrochemicals R&D Co. Ltd., Shenyang 110021, China;1. Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden;2. Center for Molecular Medicine, Karolinska University Hospital, Stockholm, Sweden;3. Department of Health Sciences Research, Mayo Clinic, Rochester, Minnesota, USA;4. Department of Psychiatry and Psychology, Mayo Clinic, Rochester, Minnesota, USA;5. Department of Gastroenterology and Hepatology, Mayo Clinic, Rochester, Minnesota, USA |
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| Abstract: | Polyclonal rabbit antiserum, raised against IAA coupled to bovine serum albumen via the indole nitrogen, was purified on a Protein A column. The immunoglobulin fraction was covalently bound to glutardialdehyde-activated silicate support and used as an immunoaffinity chromatography matrix to purify IAA in extracts from the cambial zone and shoots of Pinus sylvestris. Samples were then analysed by reverse phase HPLC with fluorescence detection. The accuracy of quantitative estimates of IAA, based on isotope dilution analyses, were verified by means of a successive approximation. The presence of IAA in the cambial tissue was further confirmed by GC/MS. |
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