Purification and separation of tomato isolectins by chromatofocusing |
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| Authors: | D C Kilpatrick J Weston S J Urbaniak |
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| Affiliation: | Regional Blood Transfusion Centre, The Royal Infirmary, Lauriston Place, Edinburgh EH3 9HB, Scotland |
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| Abstract: | Tomato lectin can be rapidly separated from a crude extract of tomato fruit proteins by chromatofocusing. The lectin is recovered from a PBE94 column in two peaks, each with a specific activity comparable to that of lectin purified by affinity chromatography on ovomucoid-Sepharose. Both isolectins consist of a single polypeptide chain (Mr 68,000) and have similar properties. |
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| Keywords: | chromatofocusing tomato lectin isolectins |
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