A rapid method for determination of acid phosphatase activity of whole yeast cells |
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| Authors: | D. Galabova B. Tuleva M. Balasheva |
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| Affiliation: | Institute of Microbiology, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria |
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| Abstract: | A simple and rapid procedure for determination of intracellular acid phosphatase activity without the need for disruption of cells is described. Candida lipolytica cell suspension was treated with 0.1% Triton X-100 for 30 min at room temperature and with intermittent shaking. The enzyme assay is carried out directly with the permeabilized cell suspension. Permeabilization of the yeast cells to p -nitrophenylphosphate by Triton X-100 provides almost 100% efficiency in determining the total acid phosphatase activity compared to results obtained with disrupted yeast cells. |
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