Metabolism and conjugation of [4-14C]progesterone by bovine liver and adipose tissues, |
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Authors: | J.D. Clemens V.L. Estergreen |
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Affiliation: | Department of Animal Sciences Washington State University, Pullman, WA 99164-6310 USA |
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Abstract: | The ability of bovine liver and fat to metabolize progesterone and also to form glucuronide conjugates with these progestins was investigated. Tissue supernatants were incubated with [4-14C] progesterone, UDP-glucuronic acid, and a NADPH generating system for 5 hr, at 37°C. Steroids were identified by thin-layer chromatography, high performance liquid chromatography, and recrystallization to a constant specific activity. The total original radioactivity which could not be removed by exhaustive ether extraction (presumptive conjugates) was 44.7 ± 14.2% in liver, 5.0 ± 3.6% in subcutaneous fat, and 3.7 ± 2.2% in kidney fat samples. Progestins identified in liver samples include 5β-pregnane-3α, 20α-diol (free and conjugate), 5β-pregnane-3α, 20β-diol (free and conjugate), 3α-hydroxy-5sB-pregnan-20-one (free and conjugate), 3β-hydroxy-5β-pregnan-20-one (free), 5β-pregnane-3, 20-dione (free), and progesterone (conjugate). Progestins identified in both the free and conjugate fractions of subcutaneous fat and kidney fat samples include progesterone, 3α-hydroxy-5β-pregnan-20-one, 20β-hydroxy-4-pregnen-3-one, and 20α-hydroxy-4-pregnen-3-one. Differences due to sex of bovine used were noted. These results confirm the ability of bovine liver to readily metabolize progesterone and form glucuronide conjugates of these compounds and suggest that adipose tissues take an active role in these actions in cattle. |
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Keywords: | 5β DHP 5β-pregnane-3, 20-dione 3α5α 3α-hydroxy-5α-pregnan-20-one 3α5β 3α-hydroxy-5β-pregnan-20-one 3β5α 3β-hydroxy-5α-pregnan-20-one 3β5β 3β-hydroxy-5β-pregnan-20-one 20α 20α-hydroxy-4-pregnen-3-one 20β 20β-hydroxy-4-pregnen-3-one 20α5α 20α-hydroxy-5α-pregnan-3-one 5β3α20α 5β-pregnane-3α, 20α-diol 5β3α20β 5β-pregnane-3α, 20β-diol 5α3β20α 5α-pregnane-3β, 20α-diol 5α3α20α 5α-pregnane-3α, 20α-diol |
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