A dual radioimmunoassay and cytosol receptor binding assay for the measurement of estrogenic compounds applied to urine,fecal and plasma samples

A technique for co-chromatography using tritiated steroids, radioimmunoassay (RIA), and a cytosol receptor binding assay was developed to examine estrogen components following elution from a reverse phase high pressure liquid chromatography (HPLC) system. Urine, fecal or plasma samples from several species of animals were assayed by this method and the results demonstrated compounds previously unreported. Two of these, one labelled E_x in primates, and one labelled E_w in birds are shown to have both cytosol receptor binding activity as well as immunoreactivity.