Biodegradation of thiocyanate using co-culture of Klebsiella pneumoniae and Ralstonia sp. |
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Authors: | Ashvini U. Chaudhari Kisan M. Kodam |
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Affiliation: | (1) Biochemistry Division, Department of Chemistry, University of Pune, Pune, 411007, India; |
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Abstract: | Thiocyanate-degrading microbial co-culture was isolated from thiocyanate-contaminated site and tested for thiocyanate degradation potential and thiocyanate-toxicity tolerance and identified as Klebsiella pneumoniae and Ralstonia sp. by 16S rDNA sequencing. The co-culture was able to degrade thiocyanate with degradation rate of 500 mg L−1d−1 at 2,500 mg L−1 thiocyanate concentration at pH 6.0 and 37oC following thiocyanate hydrolase pathway. The Haldane kinetic model elucidates the growth and thiocyanate biodegradation kinetics of the co-culture with Ki value of 1,876 mg L−1. The thiocyanate biodegradation kinetics was not affected by the additional supply of glucose. The very high activities of thiocyanate hydrolase, cyanide oxygenase, and cytochrome P-450 content during growth on thiocyanate were observed, showing the induction mechanism. |
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