High resolution G-banded chromosomes of the mouse |
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| Authors: | Jeffrey R Sawyer Martha M Moore John C Hozier |
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| Institution: | (1) Medical Genetics Laboratory, Department of Biological Sciences, Florida Institute of Technology, 32901 Melbourne, FL, USA;(2) Mutagenesis and Cellular Toxicology Branch, Genetic Toxicology Division, U.S. Environmental Protection Agency, 27711 Research Triangle Park, NC, USA;(3) Present address: Clinical and Diagnostic Foundation, Genetics Center Driscoll Children's Hospital, 78411 Corpus Christi, TX, USA;(4) Present address: Institute of Molecular Biophysics and Department of Biological Science, Florida State University, 32906 Tallahassee, FL, USA |
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| Abstract: | High resolution G-banded mouse chromosomes were prepared using an actinomycin D and acridine orange pretreatment protocol, resulting in late prophase mouse chromosomes which reveal over twice the number of bands as compared with mid metaphase. These elongated chromosomes, described here in detail and used to construct a precise schematic representation of the late prophase banding patterns, should be generally useful in high resolution mouse chromosome analysis. |
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